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rabbit polyclonal anti 53bp1 primary antibody  (Bethyl)


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    Bethyl rabbit polyclonal anti 53bp1 primary antibody
    Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing <t>53BP1+</t> DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.
    Rabbit Polyclonal Anti 53bp1 Primary Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+53bp1+primary+antibody/53BP1+Antibody/pm36682829-66-6-11
    Average 96 stars, based on 616 article reviews
    rabbit polyclonal anti 53bp1 primary antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Mouse genomic associations with in vitro sensitivity to simulated space radiation."

    Article Title: Mouse genomic associations with in vitro sensitivity to simulated space radiation.

    Journal: Life sciences in space research

    doi: 10.1016/j.lssr.2022.07.006

    Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing 53BP1+ DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.
    Figure Legend Snippet: Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing 53BP1+ DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.

    Techniques Used: Irradiation, Control

    Related Articles

    Incubation:

    Article Title: Dose, LET and Strain Dependence of Radiation-Induced 53BP1 Foci in 15 Mouse Strains Ex Vivo Introducing Novel DNA Damage Metrics
    Article Snippet: a Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720; b Namur Research Institute for Life Science, University of Namur, 5000 Namur, Belgium; c Universities Space Research Association (USRA), Columbia, Maryland; d Space Biosciences Division, NASA Ames Research Center, Mountain View, California 94035; and e Université de Nantes, 44035 Nantes, France

    Article Title: Mouse Genomic Associations with Ex Vivo Sensitivity to Simulated Space Radiation
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs #IHC-00001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 minutes (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.

    Article Title: 53BP1 Repair Kinetics for Prediction of In Vivo Radiation Susceptibility in 15 Mouse Strains
    Article Snippet: .. Briefly, fibroblasts were permeabilized with 0.1% Tritone X (Sigma-Aldricht LLC, St. Louis, MO) for 20 min and blocked with 3% bovine serum albumin (BSA; Thermo Fisher Scientific) for 60 min. Then, fibroblasts were incubated with rabbit polyclonal anti-53BP1 primary antibody (Bethyl Laboratories Inc., Montgomery, TX) at 1:400 in 3% BSA for 60 min, followed by incubation with Alexa 488 goat anti-rabbit secondary antibody (Thermo Fisher Scientific) at 1:400 in 3% BSA for 60 min. .. Nuclei were stained with DAPI (Thermo Fisher Scientific) at 1:1,000 in phosphate buffered saline (PBS) for 10 min. Plates were washed twice in 0.1% Tweent 20 (Sigma-Aldrich) between all incubations.

    Article Title: Mouse genomic associations with in vitro sensitivity to simulated space radiation.
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs E. Cekanaviciute et al. Life Sciences in Space Research 36 (2023) 47–58 #IHC-00,001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 min (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.

    Immunohistochemistry:

    Article Title: Mouse Genomic Associations with Ex Vivo Sensitivity to Simulated Space Radiation
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs #IHC-00001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 minutes (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.

    Article Title: Mouse genomic associations with in vitro sensitivity to simulated space radiation.
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs E. Cekanaviciute et al. Life Sciences in Space Research 36 (2023) 47–58 #IHC-00,001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 min (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.

    Blocking Assay:

    Article Title: Mouse Genomic Associations with Ex Vivo Sensitivity to Simulated Space Radiation
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs #IHC-00001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 minutes (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.

    Article Title: Mouse genomic associations with in vitro sensitivity to simulated space radiation.
    Article Snippet: .. Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs E. Cekanaviciute et al. Life Sciences in Space Research 36 (2023) 47–58 #IHC-00,001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS. .. Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 min (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.



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    Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing <t>53BP1+</t> DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.
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    Image Search Results


    Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing 53BP1+ DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.

    Journal: Life sciences in space research

    Article Title: Mouse genomic associations with in vitro sensitivity to simulated space radiation.

    doi: 10.1016/j.lssr.2022.07.006

    Figure Lengend Snippet: Fig. 1. Quantification of radiation-induced DNA damage in 15 mouse strains. A. Experimental design. B. Representative graph showing Foci Per Gray (FPG) and Background (BGD) phenotypes in two mouse strains (CBA and CC019) at two time points (4 h and 24 h) post-irradiation as a function of 600 MeV/n 56Fe radiation fluence (data points at 1.1 and 3 particles/100μm2). C. Representative images showing 53BP1+ DNA double stranded break repair foci in the same mouse strains at 4 h and 24 h post-irradiation with 600 MeV/n 56Fe particles at the fluence of 3 particles/100μm2 and in sham-irradiated control.

    Article Snippet: Cells were then incubated in a rabbit polyclonal anti-53BP1 primary antibody (Bethyl Labs E. Cekanaviciute et al. Life Sciences in Space Research 36 (2023) 47–58 #IHC-00,001) at 1:400 in blocking buffer (3% BSA in PBS) for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS and a subsequent incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (ThermoFisher Scientific #A11034) at 1:400 in blocking buffer for 1 hour, followed by 2 washes in 0.1% Tween-20 in PBS.

    Techniques: Irradiation, Control

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity

    doi: 10.1016/j.celrep.2019.05.058

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Primary antibody rabbit polyclonal anti-53BP1 (Abcam) and mouse monoclonal anti-PCNA (Abcam) were added for 24 hours at 4°C and secondary antibody Alexa Fluor488 goat anti-mouse (Invitrogen) and Alexa Fluor555 goat anti-rabbit (Invitrogen) were added for 1 hr at room temperature.

    Techniques: Recombinant, Transfection, Mutagenesis, Software